Jul 15, 2015 however, a direct comparison between wistar and spraguedawley sd rat as models of highfat hf diet induced obesity has not been adequately evaluated so far. Diet induced adipocyte number increase in adult rats. The red seaweed, kappaphycus alvarezii, was evaluated for its potential to prevent signs of metabolic syndrome through use as a whole food supplement. Er stress induced transcription is initiated, in part, via the splicing and activation of the transcription factor xbp1. Vd and high fat, promotes overweight by increasing fat deposits, suggestion a cause effect. International database resource for the laboratory mouse, providing integrated genetic, genomic, and biological data. Diet induced obesity in rats is associated with glucose intolerance during pregnancy but not in the nonpregnant state. Some studies have shown a relationship between obesity and infertility, but until now it remains controversial. In a first experiment, male wistar rats were fed a highfat diet hfd or standard chow sd for 15, 30 or. Highfat diets for diet induced obesity models in order to gain a greater understanding of human obesity, rats and mice are commonly used models as they will readily gain weight when provided with a highfat diet and also develop other risk factors associated with the ms. Antiobesity and uric acidlowering effect of lactobacillus plantarum. Physiogenex preclinical cro for the evaluation of your drugs efficacy in metabolic diseases keywords insulin resistance, glucose intolerance liver steatosis, hyperinsulinemic euglycemic clamp, glucoses fluxes, diabetes, pharmacology, in vivo.
These results provide novel insights into the central mechanisms underlying dex induced obesity. Thus, the aim of the present study was to investigate the effect of highfat diet induced obesity on male reproductive parameters. Dio mice are placed on high fat diet starting at 6 weeks and are packed within a 10 gram maximum weight span. Western diet induces apoptosis in the heart of a rat model with diet induced obesity the accumulation of glucose an the accumulation of glucose and fatty acid metabolites due to the imbalance of substrate uptake and oxidation is associated with contractile dysfunction in the heart. Diet induced obesity in rats is also reported to impact the brain in rats 24, as well as alter serum adipokine levels 25,26. To evaluate if obesity contributes to oa onset using a high fathigh sucrose diet induced obesity dio model with anterior cruciate ligamenttransected rats aclx. Researchers engineer metabolic pathway in mice to prevent. Using dietinduced obesity to understand a metabolic subtype. After receiving their respective diet for 10 weeks, the obese induced rats gained significantly higher body weight and bmi than the negative control rats, thus verified to be obese. Propensity to highfat dietinduced obesity in rats is. Physiogenex preclinical cro for the evaluation of your drugs efficacy in metabolic diseases keywords.
Nih07 page 1 of 3 nih07 rodent diet formulation specification open formula rat and mouse diet nih07 nsn 875097915. To evaluate if obesity contributes to oa onset using a high fathigh sucrose diet induced obesity dio model with anterior cruciate ligamenttransected rats. Wistar rats treated with a hypercaloric and hyperlipidemic diet for three months had an approximate 1. Male spraguedawley rats at 3 weeks of age were fed with a mixed normal diet of 10% fat and highfat diet of 60% fat 50. In this study we also established that sgk1 in pomc neurons is an essential regulator of systemic energy balance. Thus, rats consuming the hf diet gained increased body fat relative to rats eating either of two quite different lf diets. In summary, our results demonstrate that sgk1foxo3 signaling in pomc neurons is crucial for dex induced adiposity. Pdf modeling dietinduced obesity with obesityprone. In addition, despite the differences in the gut microbiota composition of these 2 strains, the modulation caused by hf diet was similar in both groups, except for clostridium leptum.
Diet rich in fat induced obesity not only in human but also in animals. Antiobesity and antiinflammatory effects of synthetic. Obesity and metabolic syndrome are associated with glomerulosclerosis and proteinuria, but the mechanisms are not known. This is likely to result from the additive effects of obesity and pregnancy on insulin sensitivity. The effect of diet and exercise on intestinal integrity and microbial diversity in mice. Anti obesity and antiinflammatory effects of synthetic acetic acid vinegar and nipa vinegar on highfatdiet induced obese mice skip to main content thank you for visiting. The interest in herbal medicines increases, and often they are used in combination with conventional drugs. Thus, we tried to understand the effects of obesity on adult female liver. Dietinduced obesity in rats leads to a decrease in sperm. The present study is to evaluate the antiobesity effect of lactobacillus plantarum gkm3 in highfatdietinduced obese rats and whether taking l.
In zucker rats, a genetic model of obesity, obese animals show no difference in the weights of testes or ventral prostate compared with lean rats. We performed a randomized, controlled experiment in genetically obese fatty zucker rats and diet induced obese rats to assess for behavioral and peripheral anti obesity actions of bromocriptine. Dietinduced obesity in c57bl6j mice causes increased. Amelioration of obesityrelated characteristics by a. It is intended to mimic the most common cause of obesity in humans. The zucker rat is often used in studies involving obesity. In summary, both wistar and sd rat can be used as models of hf dietinduced obesity, although most of the metabolic effects caused by hf diet were more pronounced, or earlier detected, in wistar rat.
Evidence of growth hormone effect on plasma leptin in dietinduced. Jul 10, 2012 greater diet induced obesity in rats consuming sugar solution during the inactive period. Physiogenex is a preclinical cro providing original and benchmarked animal models of obesity type 2 diabetes and related comorbidities. Effect of longterm highprotein diet on insulin secretion in. After random selection, rats and swiss mice were submitted for eight weeks to a control or highfat hf diet, as presented in table 1. Wistar rats are also used in studies where obesity is diet induced, and the results have shown that the body weight is increased. Research open access dietinduced obesity in rats leads to a. Metabolic oa, may be due to obesity related intrinsic factors but remains to be evaluated experimentally against a known oa progression model.
Dietinduced obesity in male c57bl6 mice decreases fertility. Obesity can be induced in animals by neuroendocrine, dietary or genetic changes. Modeling dietinduced obesity with obesityprone rats. Effect of longterm highprotein diet on insulin secretion in diet induced obesity rats the longterm effect of high the longterm effect of highprotein diet on glucose metabolism and insulin secretion in obese individuals remains controversial. Typically mice, rats, dogs, or nonhuman primates are used in these models. The jackson laboratory maintains an inventory of diet induced obesity dio models, and can place any strain on any fully customized diet so that your mice are ready when you need them. Protective effects of tamarillo cyphomandra betacea. Mar 11, 2011 obesity is rapidly becoming a worldwide epidemic that affects children and adults. Regarding glycemia, few studies have report ed a signi cant increase of this biochemical parameter 21,26.
Wistar rats are also used in studies where obesity is dietinduced, and the results have shown that the body weight is increased. Diet induced obese rats weighed significantly more than control rats after 24 weeks on the high fat diet. Chronic central oxytocin infusion was carried out in high fat dietinduced obese rats. However, a direct comparison between wistar and spraguedawley sd rat as models of highfat hf dietinduced obesity has not been adequately evaluated so far. Dietinduced obesity in rats leads to a decrease in.
Dietinduced adipocyte number increase in adult rats. Thus, inflammation, as well as other comorbidities, may provide a. Diet induced obesity was associated with an impairment of novel object recognition, indicating that obesity affected memory consolidation in female rats. Current study comprises usage of a diet induced obesity model by proposing high fat diet on adult female rats and examination of the livers of these obese rats under light microscope. Diets high in fat have been attributed to a host of diseases, including obesity, metabolic and inflammatory diseases. Fecal microbiota transplantation confers beneficial. However, a direct comparison between wistar and spraguedawley sd rat as models of highfat hf diet induced obesity has not been adequately evaluated so far. However, transgenic rats do exist for research purposes. Ontogeny of dietinduced obesity in selectivelybred rats. Nov 24, 2016 obesityprone op rats were maintained on a hf diet for 16 weeks to induce obesity. During the 6 weeks of obesity induction, rats were randomly divided into 2 groups and fed with either standard diet rat chow control or. Since rats and mice show a similar relationship, they are considered an appropriate model. We investigated the effect of duolac gold dg, a probiotic formulation containing 2 lactobacillus strains l.
Within scope of this aim, the obesity model was performed on animals of the study group, sprague dawley rats, by feeding them with a diet. Some rats were submitted to caloric pair feeding for eight weeks. The diet induced obesity model dio model is an animal model used to study obesity using animals that have obesity caused by being fed highfat or highdensity diets. Adult male hooded rats which were offered a mixed, high energy diet for 90 days were hyperphagic and became significantly obese compared to chow. Wistar and sd rats were assigned for 2 experimental groups for 17 weeks. Dietinduced obesity in rats is associated with glucose intolerance during pregnancy but not in the nonpregnant state.
Rats were fed a high fat diet and treated with a low dose of streptozotocin to create models of diet induced obesity or type 2 diabetes. The present study used spraguedawley rats that, when fed a highfat diet, exhibit either an obesity prone diop or obesity resistant dior phenotype, to determine whether changes in gut epithelial function and microbiota are diet or obese associated. Biometric evidence of dietinduced obesity in lewcrl rats ncbi. Rat models of dietinduced obesity and high fatlow dose. Table 1 a different types of diet used and dietinduced body and metabolic alterations dietinduced obesity. Lewcrl rats fed a 60% kcal hfd become obese, but they lack significant metabolic abnormalities frequently associated with obesity in other rat strains. Another author, studying gastric bypass models in spraguedawley rats, induced obesity with food consisting of 48% chow, 8% maize oil and 44% condensed milk for 7 to 12 weeks 48. Selective breeding for diet induced obesity and resistance in spraguedawley rats. Research article a novel wistar rat model of obesityrelated. Greater dietinduced obesity in rats consuming sugar.
Saturated fatty acids promote endoplasmic reticulum stress. Controlled highfat diet induces an obese syndrome in rats. Fasting plasma insulin and glucose levels were initially elevated in the experimental rats, but later in. Some studies have associated diet induced obesity with memory deficits in rats exposed to the object recognition and object in place tests,, corroborating. Mechanisms of the antiobesity effects of oxytocin in dietinduced. Western diet induces apoptosis in the heart of a rat model. To date, animal models of obesity based on gene modi. Rats as a model for obesity research multidisciplinary. Numerous highfat rodent diets are available from commercial vendors.
Despite its multifactorial etiology, alterations in intestinal microbiota and the immune system are frequently observed. These differences were even more marked relative to rats fed the np diet, the control traditionally used in studies of rodent dietinduced obesity. Sgk1foxo3 signaling in hypothalamic pomc neurons mediates. Gut microbiota are linked to increased susceptibility to. The aim of this study was to determine whether diet induced obesity impairs male fertility and furthermore to uncover its underlying mechanisms. The aim of this study was to examine the effect of ofs in diet. We developed a rat model of diet induced obesity that will be a powerful tool for assessment of this issue. Nutrients free fulltext kappaphycus alvarezii as a. Similarly, dio male mice exhibited no changes in the average weight of the testis or epididymis 16. Maintenance of the thyroid axis during dietinduced. Related publications genetic determinants of energy expenditure and insulin resistance in dietinduced obesity in mice.
Its impact on body weight, lipid metabolism and insulin. Highfat diet induces apoptosis of hypothalamic neurons. In the past decades, obesity and associated metabolic complications have reached epidemic proportions. In this present study, the high fat diet consists of 40% of fat of total energy. Mitochondrial proteins that regulate apoptosis and necrosis are induced in mouse fatty liver asif rashid,1 tzyychoou wu,1 chaocheng huang,1 chienhung chen,1 hui zhi lin,2 shi qi yang,2 fung ye janet lee,2 and anna mae diehl2 fatty liver is. Dietinduced obesity in c57bl6j mice causes increased renal. Jun 12, 2017 obesity is a major public health concern. While diet induced obesity has been exclusively attributed to increased caloric intake from fat, animals fed a highfat diet hfd ad libitum ad lib eat frequently throughout day and night, disrupting the normal feeding cycle.
Obesity is rapidly becoming a worldwide epidemic that affects children and adults. Rodent model for the study of obesityrelated disorders 387rev assoc med bras 2012. Stern, and jules hirsch rockefeller university, new york, new york 10021 faust, irving m. International database resource for the laboratory mouse, providing integrated. Rats were calorically restricted on an lf diet to induce 1015% loss, maintained at this reduced weight for 0, 8, or 16 weeks, then allowed to relapse with free access to lf diet. With h the diet induced obesity group, students ttest was used to assess the respective difference in the means of other groups versus h, to understand the outcomes of external treatments. Major biochemical components of dried kappaphycus are carrageenan soluble fiber 34.
Eight to nine week old male wistar rats were randomly. Highfat diet induced obesity in animal models niloofar hariri and louise thibault school of dietetics and human nutrition, mcgill university, quebec, canada epidemiological studies have shown a positive relationship between dietary fat intake and obesity. Mitochondrial proteins that regulate apoptosis and. Dietinduced obesity alters memory consolidation in female rats. However, two studies of diet induced obesity in humans and mice revealed decreases in firmicutes, which is.
Research open access dietinduced obesity in rats leads to. In a hypercaloric diet model with fat, a diet was used containing 17. Mapping of thyrotropinreleasing hormone trh neuronal systems of rat forebrain projecting to the median eminence and the ovlt. The rats were always housed in individual cages and maintained on a 12 hlightdark cycle. We challenge the current belief that obesity is a result of overnutrition by studying a rodent model of human obesity. We examined whether reversal of high fat diet, stimulating weight loss, compared to two treatments previously shown to have beneficial effects, could improve glucose utilization and peripheral neuropathy in animal models of obesity and type 2 diabetes. Cell metabolism short article hypothalamic neural projections are permanently disrupted in diet induced obese rats sebastien g. Nashfibrosis, dyslipidemia, inflammation, diabetic nephropathy and cardiovascular complications. This obese rat model is an attractive model to study further the physiologic and molecular abnormalities in gdm. To test whether obesity and metabolic diseases result from hfd or disruption of metabolic cycles, we subjected mice to either ad lib or timerestricted feeding trf. It is generally accepted that the problem of obesity reflects the merger. Research article a novel wistar rat model of obesity. Diabetes mellitus and metabolic syndrome are the common problems of the modern society. Hypoglycemic effect of goutweed aerial part tincture has been previously shown in alloxan induced.
The results demonstrate that the nutrition of rats with diet deficient in. Jun 02, 2009 researchers engineer metabolic pathway in mice to prevent diet induced obesity. Typically, chronic hfd induced obesity in both humans and rodents is marked by an increase in firmicutes and a decrease in bacteroidetes 21, 30. A free and open source software to merge, split, rotate and extract pages from pdf files. Rodent model for the study of obesity related disorders 387rev assoc med bras 2012. Obesity prone op rats were maintained on a hf diet for 16 weeks to induce obesity. It is generally accepted that the problem of obesity reflects the merger of environmental, biological, and psychosocial pressures 2.
A controlled highfat diet induces an obese syndrome in rats. Greater diet induced obesity in rats consuming sugar solution during the inactive period. Using dietinduced obesity to understand a metabolic. Interactive effects of oligofructose and obesity predisposition on gut. The purpose of this study was to determine if there is altered renal lipid metabolism and increased expression of sterol regulatory elementbinding proteins srebps in a model of diet induced obesity. This allows researchers to study a specific condition, such as obesity, without being effected by the genetic difference. Ro dent mo del for the study of obesity related disor ders rev assoc med bras 2012. We are not able to take dio orders by weight, but can assist in selecting an appropriate age to meet your weight requirement. Research article a novel wistar rat model of obesityrelated nonalcoholic fatty liver disease induced by sucroserich diet marialuizar.
Thus, male c57bl6 mice fed a highfat diet hfd for 10 weeks served as a model of diet induced obesity. Modeling dietinduced obesity with obesityprone rats ncbi. Effect of longterm highprotein diet on insulin secretion. In a first experiment, male wistar rats were fed a highfat diet hfd. For the study of these pathologies, a number of animal models have been developed. Gochujang prepared using rice and wheat koji partially alleviates. Role of vitamin d in the development of obesity scielo conicyt. Greater dietinduced obesity in rats consuming sugar solution.
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